Stability of Ascorbic Acid in Shrimp Feed during Analysis
1993 Volume 59 Issue 9 Pages 1535-1537
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1993 Volume 59 Issue 9 Pages 1535-1537
L-ascorbic acid (C1), L-ascorbyl-2-sulfate (C2), and L-ascorbyl-2-monophosphate (C3-M) were each incorporated into a laboratory-made P. monodon diet. Diets were extracted by deionized water or by 5% meta-phosphoric acid and then subjected to standing for 0, 10, 20, 30, 40, 50, and 60min before high performance liquid chromatography (HPLC) analysis. Results indicated that the laboratory diet-making process destroyed about 67-75% of C1 and 21-24% of both C2 and C3-M. C2 and C3-M were stable before the analysis after either the water or meta-phosphoric acid extraction. On the other hand, a steady decline of 27.8, 41.1, 62.6, 73.9, 81.7, and 85.7% in C1 activity was found when extracted by water and allowed to stand for 10, 20, 30, 40, 50, and 60min, respectively, before the HPLC analysis.
